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The membranes were incubated with 5% BSA at room temperature for 1 h, then with primary antibodies at 4 C overnight and second antibody successively for 1 h at room temperature, and consequently developed on the imaging system after adding ECL reagent

Mechanisms underlying treatment of UC The treatment of UC involves the modulation of various immunoinflammatory pathways, including the activation and suppression of key mechanisms such as PPAR, Nrf2, NF-kB, and macrophages, along with the regulation of the Th17/Treg balance [61]

Bee venom and melittin reduce proinflammatory mediators in lipopolysaccharide-stimulated BV2 microglia